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. 2007 Apr 26;104(19):8047–8052. doi: 10.1073/pnas.0700664104

Fig. 2.

Fig. 2.

TLR3 and 4 induces cytoplasmic localization of endogenous p27 in the presence of IFNARab. Cells stimulated in the indicated conditions were stained with anti-p27 Ab together with Alexa Fluor 594-conjugated secondary Ab. Nuclei were counterstained with DAPI. (a) Localization of p27 in Rat-1 cells stimulated with flagellin (100 ng/ml) at 0 h and 8 h with or without 30 μg/ml of IFNARab. (b) Localization of p27 in Rat-1 cells stimulated with poly (I:C) (10 μg/ml) and 8 h with/without IFNARab. (c) Localization of p27 in Rat-1 cells stimulated with LPS (50 ng/ml) at 0 h and 8 h with/without IFNARab.