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. 2007 Apr 30;104(19):8083–8088. doi: 10.1073/pnas.0702398104

Fig. 4.

Fig. 4.

Effect of paniculatumoside C (3) on accumulation of TMV RNAs and the CP. (A) Northern blot analysis of viral RNAs with the CP gene sequence as a probe. 28S rRNA stained with ethidium bromide was used as an RNA loading control. Total RNA from BY-2 protoplasts 4 h after infection with TMV treated with different concentrations of the compound. (B) ELISA analysis of TMV CP. For each concentration of the compound, the assay was repeated three times. Error bars indicate SD. (C) Effect of paniculatumoside C on expression of TMV RNAs in N. tabacum cv. K326 plants. Total RNA was isolated from leaves inoculated with TMV in the absence (lane 1) or presence (lane 2) of 50 nM of 3 and analyzed by Northen blot hybridization by using the CP gene sequence as a probe. Total RNA from the upper uninoculated leaves of the plant infected by TMV treated with 50 nM compound was analyzed in parallel (lane 3). Equal RNA loading was monitored by ethidium bromide staining of 28S rRNA.