Fig. 6.

Protection of BALB/c mice from SINV infection by paniculatumoside C (3) treatments. Mice inoculated with 20 μl of SINV (100 × LD₅₀) alone and treated with 20 μl of 3 alone at a dose 100 mg/kg BW were served as control 1 and control 2, respectively. Mice were inoculated with 20 μl (100 × LD₅₀) of SINV mixed with 20 μl of 3 at a dose of 5, 50, or 100 mg/kg BW (Test 1). Mice were treated with 3 at a dose of 100 mg/kg BW 1 and 4 h before inoculation with 100 × LD₅₀ of SINV (Test 2). Mice were administrated with 3 at a dose 100 mg/kg BW 1 or 4 h after SINV infection (Test 3). Compound 3-treated mice that survived infection were inoculated with a lethal dose (100 × LD₅₀) of SINV on day 7 after the initial viral challenge (Test 4). For each treatment, 11 mice were tested and percent survivals calculated based on the number of survival mice counted at 3, 4, 5, 6, and 7 days after the treatment.