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Western blots displaying MucA levels were reduced in the MucE-overexpressing strain PAO1VE2 as compared with the WT PAO1. The levels of AlgU and MucA protein were collected from PAO1 and PAO1VE2 cells as grown on PIA plates for the indicated amount of time. RNAP, antibodies against RNA polymerase α-subunit. The sources of antibodies are as indicated in Materials and Methods.
