Fig. 4.

Quantification of eDNA present in unwashed UAMS-1 and cidA mutant static biofilm. eDNA was isolated from untreated UAMS-1 (light gray bars) and KB1050 (cidA mutant; striped bars), DNase-treated UAMS-1 (dark gray bars) and KB1050 (gray striped bars), and PAS-treated UAMS-1 (black bars) and KB1050 (white bars) 24-hr unwashed biofilms as described in Materials and Methods. The total eDNA present in each biofilm was quantified by real-time PCR, using primer pairs specific for gyr (gyrase A), fhu (ferrichrome transport ATP-binding protein A), lys (diaminopimelate decarboxylase A), and leu (2-isopropylmalate synthase). The values are expressed as nanogram of eDNA per relative biofilm biomass, as described in Materials and Methods. Mean values from a minimum of three independent experiments, each performed in triplicate, are shown. Error bars represent the SEM.