Fig. 6.

Effects of the deletion and conversion of the E/E+ domain in CRR4. (A) Analysis of the transient increase in chlorophyll fluorescence after turning off AL. Leaves were exposed to AL (50 μmol of photons m⁻² s⁻¹) for 5 min. AL was turned off, and the subsequent change in chlorophyll fluorescence level was monitored. The fluorescence levels were normalized by Fm levels. (B) Protein blot analysis of the NDH complex. Immunodetection of NDH subunits, NdhD and NdhH, and a subunit of the cytb₆f complex, Cytf. Proteins were extracted from the thylakoid membrane fractions. Lanes were loaded with protein samples corresponding to 0.5 μg of chlorophyll for Cytf, 5 μg of chlorophyll for NdhH, and 10 μg of chlorophyll for NdhD (100%) and the series of dilutions indicated. (C) Analysis of the extent of RNA editing in the ndhD initiation codon. The editing efficiency of the ndhD-1 was analyzed as described in Materials and Methods. Ratio of clones originated from edited RNA molecule is indicated by gray bars. (D) Analysis of the extent of RNA editing in the ndhD-2 site. Ratio of clones originated from edited RNA molecule is indicated by gray bars. crr4–3+CRR4 (−E/E+), crr4–3 transformed with the CRR4 truncated in the E/E+ domain; crr4–3+CRR4 (+21E/E+), crr4–3 transformed with CRR4, in which the E/E+ domain was replaced by that of CRR21; crr21–1+CRR21(+4E/E+), crr21–1 transformed with CRR21, in which the E/E+ domain was replaced by that of CRR4.