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. 2000 May 23;97(12):6862–6867. doi: 10.1073/pnas.110053997

Figure 5.

Figure 5

Chimeric IL-5Rα/EGFR-reporter cell assay. (A) AF18748 induced activation of the IL-5Rα/EGFR chimeric receptor. Ba/F3 cells expressing a chimeric IL-5Rα/EGFR and containing a luciferase reporter gene were treated with serial dilutions of AF18748 (●), AF17121 (○), or IL-5 (□). Data points represent mean ± SEM for three separate experiments performed in triplicate. The data has been normalized to the stimulation induced by 1 μM AF18748 in each experiment. (B) Inhibition of AF18748-induced activation of the IL-5Rα/EGFR chimeric receptor by IL-5. Cells were treated with 200 pM AF18748 in the presence of increasing concentrations of IL-5. Data points represent the average of triplicate determinations from a single representative experiment.