Figure 1.

(A) A stand which holds a culture dish for Ki-exposure experiments. (a) bone cell culture and the medium; (b) 35 mm polystyrene culture dish; (c) an acrylic stand. The signs of Ki indicates that Ki-energy is applied from the fingers of a Ki-expert. (B) Photo shows how Ki-energy was applied from the fingers of Nishino inside a clean bench. (C) Schematic illustration of how osteoblastic cells were cultured. 1, Seeding of cells; 2, Proliferation in the presence of FBS until cells reach subconfluency; 3, Differentiation in the absence of FBS to reach the stage that cells can promote mineralization.