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Changes in the mRNA expressions in MC3T3-E1 cells were measured using RT–PCR. The cells were seeded, exposed to 10 min Ki-energy once, incubated for 72 h in the presence of FBS, and RT–PCR was performed. The open columns are without Ki-exposure and the filled columns are with Ki-exposure. These effects of Ki during this proliferation stage were not statistically significant. See text for details.
