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. 2004 May 12;24(19):4498–4509. doi: 10.1523/JNEUROSCI.5744-03.2004

Figure 6.

Figure 6.

The protective effects of NGF are not mediated by TrkA. Immunoblot analysis was performed to determine the effects of trophic factor withdrawal and various doses of NGF on TrkA activation. A, Immunoblot of samples from cultures subjected to 0, 1, 2, 4, and 6 hr of trophic factor withdrawal with a phosphotyrosine-specific Ab. B, Immunoblot of samples from cultures subjected to acid wash, followed by 2 hr of trophic factor withdrawal and then incubation with 0, 5, 50, 500, or 1000 ng/ml of NGF for 10 min. C, The effects of a short time course of trophic factor withdrawal on vacuole size were quantified by measuring the diameters of all visible lysosensor blue-positive vacuoles in 6-12 Purkinje neurons per treatment condition in at least three independent experiments. Usually, the total number of vacuoles measured per condition was between 100 and 200. The size distribution was graphed as a percentage of total vacuoles that were within the indicated size ranges. **, *Significant difference from 25K+S (**p < 0.01; *p < 0.05).