Figure 8.

Overexpression of a truncated p75ntr receptor lacking the ligand-binding domain induces the loss and vacuolation of Purkinje neurons. Cultures were subjected to trophic factor withdrawal or adenoviral infection with either control CMV (at 50 moi) or myristoylated intracellular domain p75ntr (at both 10 and 50 moi) adenoviruses on DIV 5. A, The cells were fixed and stained with calbindin antibodies to determine Purkinje neuron numbers after the various treatments. B, The effects of p75mICD and CMV infection at 50 moi on vacuole size were quantified by measuring the diameters of all visible lysosensor blue-positive vacuoles in 6-12 Purkinje neurons per treatment condition in at least three independent experiments. Usually, the total number of vacuoles measured per condition was between 100 and 200. The size distribution was graphed as a percentage of total vacuoles that were within the indicated size ranges. ^**, ^*Significant difference from 25K+S (^**p < 0.01; ^*p < 0.05).