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. Author manuscript; available in PMC: 2007 May 24.
Published in final edited form as: J Pharmacol Exp Ther. 2006 Jul 25;319(2):523–532. doi: 10.1124/jpet.106.109215

Figure 5. Expression of CYP2J2 splice variants.

Figure 5

Two sets of tissues, one from a postnatal (17 year old) and one from a fetal (22 wks estimated gestational age) male, were utilized for alternate splice variant analysis. Each reaction contained cDNA corresponding to 40 ng total RNA.

A: Full-length cDNA (SV1) was the major amplification product in each tissue analyzed. Minor variants were visible across the panels. M, 1 kb ladder (A); control, amplification from a plasmid mix containing the exon 2 and exon 7 deletions.

B: Graphic display of alternate splice variants identified by cloning and sequencing. Primer locations are as indicated. Boxed depict exons. Transcript-specific PCR for the exon 2 deletion variant (SV4) did not produce any visible product (not shown).