Table 2.

PCR-RFLP-based CYP2J2 genotyping assays

Allele	Enzyme	PCR in bp	Digest ºC	wild-type pattern in bp	variant pattern in bp
*2	Hpy188I	669	37	350+108+102+84+18+7	434+108+102+18+7
*3	NsiI	307	37	280 + 27	141 + 139 + 27
*4	Tsp509I	669	65	379 + 290	379 + 253 + 37
*5	MseI	244	37	214 + 30	174 + 40+ 30
*6	Tsp509I	240	65	157 + 83	240
*7	AluI	355	37	301 + 54	165 + 136 + 54
*10	ApaI	230	25	202 + 28	230

Primer sequences and other PCR details are given in Table 1. Each reaction contained 2.5U restriction enzyme and the appropriate accompanying restriction buffer that was diluted 20-fold to a final concentration 0.5x. Digestion reactions were incubated >6 hrs or overnight to ensure completeness.