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. Author manuscript; available in PMC: 2007 May 24.
Published in final edited form as: Neuron. 2006 Dec 7;52(5):789–801. doi: 10.1016/j.neuron.2006.10.024

Figure 2. Failure of BrdU-positive cells to leave the ventricular surface and PVH in MEKK4-deficient forebrain.

Figure 2

(A and B) Dams were exposed to BrdU at E14.5 and the forebrain (FB) was analyzed at E18.5.

(A) Analysis of a PVH from a MEKK4−/− “big FB” phenotype stained for BrdU (green), TUJ1 (red) and TO-PRO-3 (blue). Compared to adjacent neocortex (asterisk), heavily labeled BrdU+ cells (arrows in BrdU) co-localized with TUJ1+ (arrows) in the PVH. (A1) Higher magnification of the boxed region in A shows heavily labeled BrdU+/TUJ1+ cells (arrows) within the heterotopia. Bars = 50μm.

(B) Example of a MEKK4+/+ and MEKK4−/− with a “small FB” phenotype (sm FB) where BrdU+ cells (green) failed to leave the VZ surface compared to +/+. Nuclei were labeled with propidium iodide (red). Bar =100 μm.