Figure 7.
Effects of CTZ on PI hydrolysis in rat cerebellar granule neurons and in rat cortical astrocytes. CTZ inhibits PI hydrolysis in granule cells cultured in 5 mM K+ (K5) medium stimulated by quisqualate (A) or by ACPD (B). CTZ increases PI hydrolysis in granule cells cultured in 25 mM K+ (K25) where mGluR1 expression is very low; Quis 1 μM, NBQX 10 μM (C). CTZ had no effect on PI hydrolysis stimulated by ACPD (30 μM) in rat cortical astrocytes, which express mGluR5 but not mGluR1. Values represent means ± S.E.M. from 3 independent experiments performed in triplicate. ** p<0.01 and *** p<0.001 by Student’s t-test as compared to respective data without CTZ.