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. 1999 Jun;154(6):1643–1647. doi: 10.1016/S0002-9440(10)65419-3

Figure 1.

Figure 1.

Autoradiographs of DNA sequencing. Cycle sequencing of c-kit PT domain PCR amplified products from GCTs (designated as T) and from nonneoplastic tissue (designated as N) are shown. In nonneoplastic tissue only a wild-type sequence is present; in tumor samples mutant and wild-type sequences are coexistent, consistent with retention of one wild-type allele in the tumor cells. In all tumor samples there is a G-to-C substitution at position 2467 (solid arrows), resulting in an amino acid coding substitution (D816H). A: The two components of a mixed ovarian GCT, a yolk sac tumor component (designated as T1) and a dysgerminoma component (designated as T2), were sampled separately. Both tumor components contain the G-to-C substitution resulting in the D816H mutation (solid arrow). In addition, there is a C-to-T substitution at nucleotide position 2469 (open arrow), which does not result in a change in amino acid coding. B: Results from a seminoma showing the G-to-C substitution at position 2467 that results in the D816H amino acid missense mutation.