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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Dev Biol. 2006 Sep 16;302(1):230–242. doi: 10.1016/j.ydbio.2006.09.023

Figure 6.

Figure 6

Tbx5 is localized to the cytoplasm when expressed in coronary smooth muscle. Cryosections from chicken embryos were processed for immunohistochemistry using antisera specific for Tbx5, LMP4 or caldesmon. (A-D) Cryosections from HH29 chick embryos were stained for Tbx5 (A) and smooth muscle marker caldesmon (B). Merged image (C). Overlay with DAPI (D). (E-H) A similar section was stained for LMP4 (E) and caldesmon (F). Merged image (G). Overlay with DAPI (H). (I) A region of the left atrio-ventricular groove from a HH36 heart is shown, containing a portion of the left atria and a coronary vessel (arrow). (J-M) Enlarged view of the coronary vessel stained for Tbx5 (J). Overlay of Tbx5 with DAPI (K). Caldesmon (L). Merge between Tbx5 and caldesmon (M). (N-Q) An adjacent section was stained for LMP4 (N). Overlay of LMP4 with DAPI (O). Caldesmon (P). Merge between LMP4 and caldesmon (Q). (R-Y) A coronary vessel located within the myocardium of a HH39 heart is shown. (R-U) Tbx5 (R), overlay with DAPI (S), caldesmon (T) and merge between Tbx5 and caldesmon (U). (V-Y) An adjacent section is shown, co-stained for Tbx5 (V) and LMP4 (W). Merged image (X). Overlay with DAPI (Y). epi=epicardium; myo=myocardium; LA=left atria; LV=left ventricle; OT=outflow tract. Scale bars=20μm.