Dissection of the B6.9 fragment. Top: Summary of fragments tested using lacZ reporters. Bottom: X-Gal stained eye-antennal, leg, and wing discs showing the expression patterns driven by each fragment. B6.9 drives expression in the distal antenna, but not in leg or wing discs. In contrast, the S4.9 and E2.0 subfragments drive expression strongly in both antennal and leg discs. E2.0 also drives expression in a ventral stripe in the antennal disc and in the dorsal hinge region of the wing disc. S4.9 shows very weak expression in this same region of the wing disc. The leg and antennal disc expression of E2.0 prove to be largely separable, with the 522 and 531 fragments driving expression primarily in the distal antenna and leg, respectively. The separation is not complete, however, with 522 driving weak mottled expression in the distal leg, and 531 driving expression in the aristal region and in a ventral stripe in the antennal disc. 522 and 531 also drive expression in wing discs in the lateral and dorsal hinge regions, respectively. The E1.6, E1.9, EX1.9, 542 and 554 fragments do not drive expression in antennal or leg discs.