Figure 5.

Representative autoradiographs showing levels of the 67-kd EBP during the chase after metabolic pulse-labeling with radioactive serine. Results indicate that both normal and Hurler fibroblasts initially synthesize comparable amounts of EBP, isolated by elastin affinity columns. In contrast to normal cells, which retain the majority of the labeled EBP, Hurler fibroblasts steadily lose this newly produced protein, which can be detected in the increased amounts in the respective conditioned media during the chase.