Figure 2.

Localization of Bax by immunofluorescence in the human retina. A and D: Bax immunoreactivity is present in ganglion cells (arrow) and inner nuclear layer (INL) in the retina of a 68-year-old male with an 11-year history of diabetes (A) and a 71-year-old male nondiabetic (D). The outer nuclear layer (ONL) is negative. B and E: Bax immunoreactivity in the endothelial lining and medial layer of a large vessel (arrowhead), as well as in cells of the inner nuclear layer in the retina of a 61-year-old female with a 7-year history of diabetes (B) and a 56-year-old male nondiabetic (E). C: Negative control obtained by using nonimmune rabbit serum on a retinal section from the donor shown in B. Scale bars, 22 μm.