Figure 4.

Representative immunoblot comparing Bax levels in human whole retina (R) and vessels (V) isolated from the contralateral retina by hypotonic lysis. In the specimens of both a 71-year-old nondiabetic (left three lanes) and a 71-year-old diabetic donor, Bax levels per microgram of protein are in vessels approximately half those in whole retina. The Bax signal, more intense in the whole retina of the diabetic donor, was also more intense in the vessels of his contralateral retina when compared with the respective specimens of the nondiabetic donor.