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Western blotting for HGF receptor expression using subcellular protein preparations of selected tumor samples with a C-terminal anti-HGF receptor antibody C-28. Note the full-length 140-kd β-chain of the HGF receptor located mainly in the membranous fraction (Mem) and the shorter 85-kd fragment located predominantly in the cytoplasmic fraction (Cyt). pAb, polyclonal antibody; MAb, monoclonal antibody.
