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. 2000 Mar;156(3):1073–1083. doi: 10.1016/S0002-9440(10)64975-9

Figure 4.

Figure 4.

Peritoneal inflammatory cells of gal3−/− mice exhibit a reduced NF-κB response. Nuclear extracts were obtained from peritoneal inflammatory cells and the κB binding activity was determined by EMSA. A: Peritoneal cells from gal3−/− and gal3+/+ mice were obtained 2 days after thioglycollate broth treatment. Each lane represents results from a single mouse. Similar results were obtained from three separate experiments. B: Peritoneal cells from untreated or thioglycollate-treated mice (1 or 2 days after the treatment) were obtained by lavage and cells from three mice for each group were pooled. They were incubated in RPMI (5 × 10 6 cells/ml) at 37°C in the absence or presence of recombinant TNF-α (40 ng/ml) for 40 minutes. The arrowheads mark the positions of DNA-protein complexes specific for NF-κB.