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. 2000 Mar;156(3):807–812. doi: 10.1016/S0002-9440(10)64948-6

Figure 1.

Figure 1.

Reverse transcription-polymerase chain reaction assay. a: RT-PCR with sense primer API2/S897–916 and antisense primer MALT1/AS1030–1049 demonstrated specific fragments in cases 1–5, 7, 8, and 13. The remaining cases did not show any amplified fragments. The size of the amplified fragments is 1487 bp for cases 1, 5, 7 and 13, 1184 bp for cases 2 and 4, 1466 bp for case 3, and 1211 bp for case 8. b: The same cases also showed amplification when analyzed with sense primer API2/S1203–1222 and antisense primer MALT1/AS1030–1049. The amplified fragments are 306 bp smaller than those shown in a. The size of the fragments is 1181 bp for cases 1, 5, 7, and 13, 878 bp for cases 2 and 4, 1160 bp for case 3, and 905 bp for case 8. c: RT-PCR with a β-actin primer pair that amplifies 512 bp is shown for RNA quality control. dH2O represents PCR without any first-strand cDNA. Markers are λHindIII and φHaeIII.