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A: The amino acid sequence of the hfgl2 protein. Bolded amino acids refer to glycosylation sites. The underlined amino acids correspond to the well-conserved fibrinogen related domain. B: Western blot of human prothrombinase, hfgl2, expressed in CHO cells. Lane 1, hfgl2 reduced by dithiothreitol (DTT); lane 2, CHO cells transfected with vector alone. Twenty μg of total protein was added to each lane.
