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. 2000 Apr;156(4):1275–1287. doi: 10.1016/S0002-9440(10)64998-X

Figure 1.

Figure 1.

A: Time-dependent induction of Cox-2 mRNA by LPS. Lungs undergoing buffer perfusion for 2 hours in the absence of LPS (control; Co) and lungs perfused in the presence of 10,000 ng/ml LPS for 1 or 2 hours were snap-frozen, tissue was homogenized, and expression of Cox-1 and Cox-2 mRNA was detected by Northern blot analysis (examples given in right panel). To compare different blots, expression of Cox-2 mRNA in control lungs was set at 1. After 1 and 2 hours, Cox-2 mRNA expression was increased to approximately fivefold values (means ± SE for n = 3 lungs per group given in the left panel). B: Concentration-dependent increase in Cox-2 mRNA. Lungs undergoing buffer/plasma perfusion for 2 hours in the absence of LPS (control; Co) and lungs perfused in the presence of 1.5% plasma and 50, 1000, and 10,000 ng/ml LPS for 2 hours were snap-frozen, homogenization of tissue was performed, and expression of Cox-2 mRNA was detected by Northern blot analysis (examples given in right panel). To compare different blots, expression of Cox-2 mRNA in control lungs was set at 1 (left panel; means ± SE of n = 3 blots each).