Figure 4.

UCP3 mRNA and protein measurements. (A and B) The Northern analysis for UCP3 mRNA in skeletal muscle reflects a graded increase in expression from 1 to 8 days of T₃ treatment vs. control. (C and D) The Western analysis for UCP3 protein in skeletal muscle reflects an immediate increase in UCP3 content after 1 day of T₃ treatment and no further increase thereafter. Data are presented as means ± SEM. p, purified protein; con, control; 1 day, 3 day, or 8 day, 1, 3, or 8 days of T₃ treatment. *, P < 0.05 vs. baseline; **, P < 0.01 vs. baseline; ***, P < 0.0001 vs. baseline; †, P < 0.05 vs. baseline.