Figure 6.

PMN migration across inverted monolayers rescued or not with CFTR, in response to adherent PAO1. Confluent CF₁₅ cells were infected or not with Ad RSV CFTR or Ad RSV βgal (MOI 500), before being incubated with PAO1 on their apical surface. The absolute values of three experiments done in quadruplicate were pooled. Data are means ± SEM of the 12 samples pooled. In parallel, monolayers that were neither infected with viruses nor challenged with PAO1 were assessed for migration in response to 50 nmol/L fMLP as a means of comparison. In the Ad βgal-infected groups, X-Gal staining revealed between ∼40% and ∼80% of blue cells and, in the Ad CFTR-infected groups, the response to low Cl⁻ and forskolin varied between 28 and 57 μA/cm².