Figure 2.
Mannose inhibition of endothelial iC3b deposition. iC3b deposition on hypoxic (24 hours) HUVECs reoxygenated (3 hours) in the presence of 30% HS treated with 0, 3, 30, or 300 mmol/L D-mannose or L-mannose (300 mmol/L) was measured by ELISA. D-mannose, but not L-mannose, reduced endothelial iC3b deposition in a dose-dependent manner. Similarly, GlcNAC (30 mmol/L) inhibited iC3b deposition. Data are normalized to hypoxic HUVECs reoxygenated in 30% HS (vehicle; OD405 = 0.14 ± 0.01). n = 3; error bars = SE; *P < 0.05 compared to vehicle.