Vessels were counted on days 5, 6, 7, 9, and 14 in incisional wounds (PPI) and in punch biopsy wounds (PSI) from 4 to 7 square grids (area = 0.16 mm2) in a magnification field (40×), and the mean value score and SD were calculated. Only vessels with a lumen or consisting of more than a single endothelial cell were calculated. The granulation tissue did not develop into the wound area until day 4 to 5.