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Methylation-specific PCR assay of the p16INK4a gene. Agarose gel electrophoresis of two representative cases (MF78, MF91) is shown at the plaque (PL) and tumoral (T) stages. Primer sets used for amplification are designated as unmethylated (U) or methylated (M). The Raji cell line and peripheral blood lymphocytes (PBLs) serve as positive controls for methylated (M) and unmethylated (U) p16 alleles, respectively.
