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Reverse transcriptase-polymerase chain reaction for C-terminal agrin (A), N-terminal agrin (B), utrophin (C), and dystroglycan (D) on cDNA from cortex, glomeruli, and cultured podocytes and mesangial cells. M50 bp marker. Lanes 1 and 2, kidney cortex; lanes 3 and 4, glomeruli; lanes 5 and 6, podocytes; lanes 7 and 8, mesangial cells; lane 9, buffers only. Lanes 1, 3, 5, and 7: cDNA (reverse-transcribed RNA); lanes 2, 4, 6, and 8: nonreverse-transcribed RNA.
