Table 1.

Primers and Probes Used in RT-PCR and Southern Blot

	Reference no.	GenBank no.		Oligo sequence 5′→3′	Base pair number	Fragment length	MgCl2 concentration	Annealing temp. (°C)
Agrin N-terminus	38, 39	M64780	Forward primer	GCC GTA TAG GTG CAA CCC G	998-1016	101	2.5	62
			Reverse primer	TAC GGA GTT AAA CTG GCA GGT CT	1098-1076
			Probe	AAA GTA CGC TCT GGT CAA TGC CAA	1033-1056
Agrin C-terminus	38, 39	M64780	Forward primer	TTC GAA TCA GGG CTC ACA GG	5744-5763	101	0.5	62
			Reverse primer	GTC CAG TTG CGT GGC ACC	5844-5827
			Probe	AGC CCC TGT GAC TGG ATC TTC C	5799-5820
Dystroglycan	40	H35660	Forward primer	TTT GGA AGA AAC CAT TTT GAG CAT	95-118	101	1.0	54
			Reverse primer	CAG GGA AGG GAT ACA TTA TTG CA	195-173
			Probe	GTA CCT TTT AGG GAG GAA TGC CTT TT	148-173
Utrophin	41, 42	Y12229/	Forward	GCC CAC AAT GAC ATA TTT AAA AGC AT	7492-7517 (Y12229)	154	1.0	54
		X69086	primer		7501-7526 (X69086)
			Reverse	CCC TGA TGC TAG CAG ATT TTG C	7645-7624 (Y12229)
			primer		7654-7633 (X69086)
			Probe	CGG CAG AAG ATG GTG AAA GCT CT	7528-7550 (Y12229)