Table 3.
Antibodies Used to Phenotype Cultured Podocytes and Mesangial Cells
| Antibody | Antigen | Reference no. | Dilution IF | Conjugate | Dilution |
|---|---|---|---|---|---|
| C19* | WT-1 | 50 | Swine anti-rabbit IgG-FITC† | 40 | |
| G1 | Synaptopodin | 49 | 1 | Goat anti-mouse IgG-cy3∥ | 200 |
| 5A | Podocalyxin | 50 | 10 | Goat anti-mouse IgG-cy3∥ | 200 |
| 27A | O-acetylated ganglioside | 50 | 10 | Goat anti-mouse IgG-cy3∥ | 200 |
| V9† | Vimentin | 800 | Goat anti-mouse IgG-cy3∥ | 200 | |
| D33† | Desmin | 400 | Goat anti-mouse IgG-cy3∥ | 200 | |
| OX7‡ | Thy1.1 | 400 | Goat anti-mouse IgG-cy3∥ | 200 | |
| 1A4§ | Smooth muscle actin | 5000 | Goat anti-mouse IgG-cy3∥ | 200 | |
| Anti-Factor VIII/p | Factor VIII | 300 | Swine anti-rabbit IgG-FITC† | 40 | |
| IIH6 | α-dystroglycan | 45 | 1 | Goat anti-mouse IgG-cy3∥ | 200 |
*Santa Cruz Biotechnology, Heidelberg, Germany.
†DAKO, Glostrup, Denmark.
‡PharMingen, San Diego, CA.
§Sigma, St. Louis, MO.
¶Central Laboratory Blood Transfusion Service, Amsterdam, The Netherlands.
∥Biotrend, Cologne, Germany.