Table 4.
Expression of Various Antigens in Glomeruli of Normal Rats and on Cultured Podocytes and Mesangial Cells
| Antibody | Antigen | In situ staining | In vitro staining | ||||
|---|---|---|---|---|---|---|---|
| GVEC | GPEC | MC | Endo | GVEC | MC | ||
| C19 | WT1 | + | − | − | − | + | ± |
| G1 | Synaptopodin | + | − | − | + | + | − |
| 5A | Podocalyxin | + | − | − | + | − | − |
| 27A | O-acetylated ganglioside | + | − | − | − | − | − |
| V9 | Vimentin | + | + | − | + | + | + |
| D33 | Desmin | − | − | + | − | − | + |
| OX7 | Thy 1.1 | − | − | + | − | − | + |
| 1A4 | Smooth muscle actin | − | − | + | − | + | + |
| Anti-Factor VIII | Factor VIII | − | − | − | + | − | − |
| IIH6 | α-dystroglycan | + | − | − | − | ± | + |
GVEC, glomerular visceral epithelial cell or podocyte; GPEC, glomerular parietal epithelial cell; MC, mesangial cell; Endo, endothelial cell.
+, positive staining; −, negative staining