FIGURE 2. Changes in sequence-specific NF-κB binding activity in IEC-6 cells exposed to TCDCA. A, Representative audiograms of NF-κB binding. Cells were grown in DMEM with 5% dialyzed FBS with and without TCDCA for 6 days. Nuclear extracts were prepared, and electrophoretic mobility shift assay (EMSA) was performed by using 10 μg of nuclear proteins and 0.035 pmol of ³²P-end-labeled oligonucleotides containing a single NF-κB binding site. Position of specifically bound DNA-protein complex is indicated. B, Effects of unlabeled NF-κB oligonucleotide as a cold competitor on NF-κΒ binding activity in the presence of 0.5 mmol/L TCDCA as measured by EMSA.