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. 2007 Jun;18(6):2072–2080. doi: 10.1091/mbc.E06-10-0968

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© 2007 by The American Society for Cell Biology

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Figure 6. — Functional effects of 49- and 42-kDa Sgk1 isoforms on ENaC current. Clones of A6 cells expressing 49- or 42-kDa Sgk1 isoforms ± activating mutation S422D under control of tetracycline. (A and D) Time course of Sgk1 expression assessed by Western blotting at the indicated times. (B and E) Time course of amiloride-sensitive Isc after induction with tetracycline at time 0 (●) and quantification by densitometry of western blots at the corresponding time points (○). (C and F) Immunofluorescence of each clone grown of Transwells ± tetracycline with anti-HA antibody.