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Effects of 49- and 42-kDa Sgk1 isoforms on Foxo3a translocation. (A) Cells transfected with FoxO3a-V5 alone or with Sgk1 49 or 42 isoforms were examined with anti-V5 antibody. Serum was removed 16 h before experiments. Quantification of FoxO3a localization in nucleus (hatched), cytoplasm (black), and nucleus+cytoplasm (white) in 100–150 cells. (B) Identification of Sgk1 isoforms (green) with anti-FLAG and FoxO3a (red) with anti-V5.
