Figure 1.

Loss of Rga4p alters cellular dimensions. (A) a, wild-type 972. b, rga4Δ (FV513). c, rga4Δ strain expressing HA-Rga4p under the control of the nmt1 promoter (FV531), grown in the absence of thiamine for 16 h at 32°C. Cells were stained with calcofluor. Bar, 10 μm. (B) Effect of rga4 deletion on cell length and cell diameter at division expressed as average ± SD. Twenty-five cells were measured for each condition. (C) Localization of chromosomally tagged Rga4-GFP (FV781) and Rga4-HA (FV532). a and b, Rga4-GFP; d and e, corresponding DIC images. c, Rga4-HA; f, corresponding DAPI visualization. Note that in d and e overall cell dimensions are altered by methanol fixation. Bar, 10 μm.