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. 2007 Jun;18(6):2090–2101. doi: 10.1091/mbc.E06-09-0883

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© 2007 by The American Society for Cell Biology

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Figure 6. — Overexpression of the N-terminus of Rga4p induces actin cable formation in a For3p-dependent manner. (a) Wild-type cells carrying an empty Rep3X plasmid (FV800). (b) Wild-type cells expressing full length HA-Rga4p (FV801). (c) Wild-type cells expressing HA-Rga4p-ΔGAP (FV804). (d) Wild-type cells expressing HA-Rga4p- ΔN (FV802). (e) rga4Δ cells carrying an empty Rep3X plasmid (FV416). (f) rga4Δ cells expressing full length HA-Rga4p (FV531). (g) rga4Δ cells expressing HA-Rga4p-ΔGAP (FV575). (h) rga4Δ cells expressing HA-Rga4p- ΔN (FV574). (i) for3Δ cells carrying an empty Rep3X plasmid (FV605). (j) for3Δ cells expressing HA-Rga4p (FV606). (k) for3Δ cells expressing HARga4p- ΔGAP (FV604). (l) for3Δ cells expressing HA-Rga4p-ΔN (FV603). (m) tea4Δ cells carrying an empty Rep3X plasmid (FV759). (n) tea4Δ cells expressing HA-Rga4p (FV760). (o) tea4Δ cells expressing HA-Rga4p-ΔGAP (FV761). (p) tea4Δ cells expressing HA-Rga4p-ΔN (FV762). Cells were grown for 16 h at 32°C in the absence of thiamine. Bar, 10 μm.