Figure 7.

Distribution of PC labeled by mouse anti-PC antibody (IgM) and colloidal gold (5-nm)-conjugated goat anti-mouse Ig antibody. (A) The labeling of PC in the normal mouse fibroblast was observed as clusters in the E face, which was similar to that seen in liposomes and in other cell types (Fujimoto et al., 1996). (B) The K-function analysis of 10 areas (1 × 1 μm) showed that the labeling was clustered in the normal mouse fibroblast. The clustering was observed similarly in cells depleted of cholesterol, but it decreased in chilled cells.