Table 1.

Genotypes and phenotypes of progeny trans-heterozygous for RNAi and da-GAL4 transgenes

Genotypes of trans-heterozygous progeny X/X; 2/2; 3/3 (insertion chromosome and site of insertion)	Percent Nopp140 transcript levels	Phenotype of trans-heterozygous progeny
w1118/w−; +/+; +/da-GAL4	100	Normal development and normal adult progeny
w1118/w−; +/+; TCom.B1(B2)/da-GAL4(Chromosome 3R at 107247)	∼70	100% pupal eclosion; 95% of the adult progeny showed combinations of wing, leg, or tergite defects
w1118/w−; +/+; TCom.C3/da-GAL4 (Chromosome 3R at 4806161)	∼50	100% early pupal lethality
w1118/w−; TCom.C4/+; TCom.C4/da-GAL4 (chromosome 2, site not determined) (chromosome 3R at 4806162)	∼30	Severe developmental delay; 100% third instar lethality
w1118/w−; PCom.K1(K4)/+; +/da-GAL4 (chromosome 2L at 13211775)	∼65	Severe developmental delay, 100% pupal lethality