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. 2000 Jun 6;97(12):6890–6895. doi: 10.1073/pnas.97.12.6890

Figure 1.

Figure 1

Structural relationship between AYVV DNA A, DNA β and recombinant def17. (A) The position and orientation of DNA A virion-sense (V) and complementary-sense (C) genes is shown in relation to the stem-loop sequence containing the conserved nonanucleotide TAATATT↓AC. Regions of the recombinant def17 derive from DNA A (black) and DNA β (gray) sequences, the coordinates of which are indicated. Nucleotide numbering starts from the putative nick site (↓) within the conserved nonanucleotide. The double-headed arrow demarcates the region of DNA β showing homologies to TLCV satellite DNA. The positions of primers used to PCR-amplify DNA β fragments and the SpeI site used to clone DNA β are indicated. (B) The program frames (13) was used to predict DNA β ORFs, starting from an in-frame ATG triplet, that occur in the virion-sense (top) and complementary-sense (bottom) strands. The program testcode predicted two potential coding regions (black ORFs). Nucleotide numbering of DNA β follows the accepted convention for geminivirus DNAs and begins immediately downstream of the putative nick site within the conserved nonanucleotide.