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Detection of viral DNAs in agroinoculated N. benthamiana plants. Samples (5 μg of nucleic acids) were extracted 15 days p.i. from individual plants agroinoculated with combinations of DNA A, DNA β, and DNA 1 as indicated. Samples were fractionated by using agarose gels in TNE buffer, and blots were screened by using probes specific for DNA A (Top), DNA 1 (Middle), and DNA β (Bottom). The position of single-stranded DNA (ss) is shown.
