Figure 5.

Detection of viral DNAs in agroinoculated N. benthamiana leaf disks. Samples were extracted 3, 5, and 7 days p.i. from leaf disks agroinoculated with combinations of DNA A, DNA β, and DNA 1 as indicated. A nucleic acid sample from a symptomatic N. benthamiana plant infected by agroinoculation with DNA A and DNA β is in lane M. Equivalent amounts (5 μg) of nucleic acids were loaded in each lane. Samples were fractionated by using agarose gels in TNE buffer, and blots were screened by using probes specific for DNA A (Top), DNA β (Middle), and DNA 1 (Bottom). The position of single-stranded DNA (ss) is shown, and residual inoculum DNA is associated with the multiple bands at the top of each blot.