Figure 6.

Immunoprecipitation of DNA β. Nucleic acids were extracted either from symptomatic N. benthamiana plants that had been agroinoculated with DNA A and DNA β (lanes 1 and 2) or from virions that had been immunoprecipitated by using African cassava mosaic virus polyclonal antiserum (lanes 3 and 4). Duplicate samples were either untreated (lanes 1 and 3) or treated with mungbean nuclease (lanes 2 and 4) before fractionation on an agarose gel in TNE buffer. Blots were screened by using strand-specific probes synthesized in vitro from pBS-AYVVβ using the T7 or T3 promoter as indicated. The positions of single-stranded (ss), supercoiled (sc), open-circular (oc), and dimeric single-stranded (ssd) DNAs are shown.