Figure 6.

GIT1 and βPIX control permeability in vitro. (A) Cells transfected as in Figure 4C were plated on filters with 3-μm pores at confluent density. Cultures were stimulated with VEGF, and permeability to HRP was assayed as described in Materials and Methods. (B) HUVECs were transfected with siRNA oligonucleotides specific to GIT1 or with control, scrambled siRNA. Total cell lysates at 72 h were analyzed for GIT1 expression by Western blotting (top). Cells on filters were analyzed for permeability to HRP after treatment with 50 ng/ml VEGF, 10 μM histamine, or 0.2 U/ml thrombin.