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Effect of Sse1 ATPase domain point mutations. (A) Strain BY241 [URE3-1] with overproducted wild-type Sse1p, Sse1-K69Q, Sse1-G233D, or vector streaked on ½ YPD (figures indicate relative prion loss). (B) Levels of normal and mutant Sse1p overproduction, measured with anti-Sse1 antibody and compared with vector alone (WT). The loading control is a nonspecific band recognized by anti-Sse1 antiserum, and the results were confirmed by Ponceau S staining.
