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Activation of Arf1 and Arf6 by ARNO and EFA6 in cells. Arf1-HA or Arf6-HA were expressed in HeLa cells either alone or in the presence of Flag-ARNO or Flag-EFA6. The fraction of Arf1 (■) or Arf6 (▩) that was GTP-bound was determined using a GGA3-GAT pulldown assay as described in Materials and Methods. Shown are the means and SD of triplicate experiments.
