Figure 1.

The stable ATP analogue ATPγS induces a nuclear export of HuR in hMC (left). Indirect immunofluorescence was applied to visualize changes in the subcellular localization of HuR by ATP (note the different magnification in the top and bottom panels). Quiescent hMC were stimulated for 4 h with either vehicle or with 30 μM ATPγS as indicated before cells were fixed and stained with an anti-HuR antibody and subsequently with anti-mouse-Alexa 488 used as a secondary antibody. Data are representative of two independent experiments giving similar results.